Volume 2, Issue 1 (Winter 2018)                   Multidiscip Cancer Investig 2018, 2(1): 13-21 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abodolmaleki P, Haghighat N. The Effects of Synthesized Superparamagnetic Iron Oxide Nanoparticles and Electromagnetic Field on Cell Death of MCF-7 Breast Cancer Cell Line. Multidiscip Cancer Investig. 2018; 2 (1) :13-21
URL: http://mcijournal.com/article-1-64-en.html
Abstract:   (140 Views)
Introduction: Iron oxide nanoparticles, due to very small dimensions and superparamagnetic properties, are proposed as a potential element for many medical applications. The current study aimed at evaluating the synthesis and characteristics of superparamagnetic iron oxide nanoparticles (SPION) and the cell death induced by SPION in the presence of electromagnetic field (EMF).
Methods: The superparamagnetic nanoparticles were initially synthesized using the chemical co-precipitation method. Nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential. A vibrating-sample magnetometer (VSM) was used to measure the magnetic properties of the nanoparticle. The human MCF-7 breast cancer cell line was treated with different concentrations of SPION in the absence and presence of a 50-Hz EMF for 24 and 48 hours. The cytotoxicity and cell viability percentage in the treated cells were performed by the MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.
Results: The results obtained from DLS and TEM indicated that the synthesized nanoparticles had an average size below 30 nm and particles were consequently superparamagnetic. The results obtained using VSM also confirmed the superparamagnetic behavior of nanoparticles. The MTT assay revealed that high concentrations of SPION induced cell death in MCF-7 cells. In the groups treated with EMF+SPION, cell death increased sharply, compared with the groups treated with each treatment alone (P ≤0.05). 
Conclusions: In conclusion it seems that a 50-Hz EMF in the presence of SPION led to cell death due to local heating.
Full-Text [PDF 959 kb]   (17 Downloads)    
Type of Study: Original/Research Article | Subject: treatment
Received: 2017/08/17 | Accepted: 2017/12/25 | ePublished: 2018/01/1

1. Zaitsev VS, Filimonov DS, Presnyakov IA, Gambino RJ, Chu B. Physical and Chemical Properties of Magnetite and Magnetite Polymer Nanoparticles and Their Colloidal Dispersions. J Colloid Interface Sci. 1999;212(1):49-57. [DOI]
2. Schlorf T, Meincke M, Kossel E, Glüer CC, Jansen O, Mentlein R. Biological properties of iron oxide nanoparticles for cellular and molecular magnetic resonance imaging. Int J Mol Sci. 2010;12(1):12–23. [DOI] [PubMed]
3. Li L, Jiang W, Luo K, Song H, Lan F, Wu Y et al. Su-perparamagnetic iron oxide nanoparticles as MRI contrast agents for noninvasive stem cell labeling and tracking. Theranostics. 2013;3(8):595–615. [DOI] [PubMed]
4. Chen B, Wu W, Wang X. Magnetic iron oxide nanoparticles for tumor-targeted therapy. Curr Cancer Drug Targets. 2011s;11(2):184–9. 94328475 [DOI] [PubMed]
5. Hamaguchi T, Kato K, Yasui H, Morizane C, Ikeda M, Ueno H, et al. A phase I and pharmacokinetic study of NK105, a paclitaxel-incorporating micellar nanoparticle formulation. Br J Cancer. 2007;97(2):170-6. . [DOI] [PubMed]
6. Shi Y, Zhou L, Wang R, Pang Y, Xiao W, Li H, et al. In situ preparation of magnetic nonviral gene vectors and magnetofection in vitro. Nanotechnology. 2010;21 (11):115103. [DOI]
7. Kami D, Takeda S, Itakura Y, Gojo S, Watanabe M, Toyoda M. Application of magnetic nanoparticles to gene delivery. Int J Mol Sci. 2011;12(6):3705–22. [DOI] [PubMed]
8. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA Cancer J Clin. 2016;66(1):7–30. PMID: [DOI] [PubMed]
9. Zeichner SB, Terawaki H, Gogineni K. A review of systemic treatment in metastatic triple-negative breast cancer. Breast Cancer (Auckl). 2016;10:25–36. [DOI] [PubMed]
10. Wang Y, Zhang T, Kwiatkowski N, Abraham BJ, Lee TI, Xie S et al. CDK7-dependent transcriptional addiction in triple-negative breast cancer. Cell. 2015; 163(1): 174–86. [DOI] [PubMed]
11. Musgrove EA, Sutherland RL. Biological determinants of endocrine resistance in breast cancer. Nat Rev Cancer. 2009;9(9):631–43. [DOI] [PubMed]
12. Cleator SJ, Ahamed E, Coombes RC, Palmieri C. A 2009 update on the treatment of patients with hormone receptor-positive breast cancer. Clin Breast Cancer. 2009;9 Suppl 1:S6–17. [DOI] [PubMed]
13. Phillips JL, Singh NP, Lai H. Electromagnetic fields and DNA damage. Pathophysiology. 2009;16(2-3):79–88. [DOI] [PubMed]
14. Pall ML. Electromagnetic fields act via activation of voltage-gated calcium channels to produce beneficial or adverse effects. J Cell Mol Med. 2013;17(8):958–65. [DOI] [PubMed]
15. Barbault A, Costa FP, Bottger B, Munden RF, Bomholt F, Kuster N, et al. Amplitude-modulated electromagnetic fields for the treatment of cancer: discovery of tumor-specific frequencies and assessment of a novel therapeutic approach. J Exp Clin Cancer Res. 2009;28:51. [DOI] [PubMed]
16. Williams CD, Markov MS, Hardman WE, Cameron IL. Therapeutic electromagnetic field effects on angiogenesis and tumor growth. Anticancer Res. 2001;21(6A):3887-91. [PubMed]
17. Jadhav NV, Prasad AI, Kumar A, Mishra R, Dhara S, Babu KR, et al. Synthesis of oleic acid functionalized Fe3O4 magnetic nanoparticles and studying their interaction with tumor cells for potential hyperthermia applications. Col-loids Surf B Biointerfaces. 2013;108:158-68. [DOI] [PubMed]
18. Pollert E, Veverka P, Veverka M, Kaman O. Search of new core materials for magnetic fluid hyperthermia: preliminary chemical and physical issues. Prog. Solid State; 2009.
19. Granov AM, Muratov OV, Frolov VF. Problems in the Local Hyperthermia of Inductively Heated Embolized Tissues. Theor Found Chem Eng. 2002;36(1):63–6. [DOI]
20. Huber DL. Synthesis, properties, and applications of iron nanoparticles. Small. 2005;1(5):482–501. [DOI] [PubMed]
21. Krishnan KM. Biomedical nanomagnetics: a spin through possibilities in imaging, diagnostics, and therapy. IEEE Trans Magn. 2010;46(7):2523–58. [DOI] [PubMed]
22. Lu AH, Salabas EL, Schüth F. Magnetic nanoparticles: synthesis, protection, functionalization, and application. Angew Chem Int Ed Engl. 2007;46(8):1222–44. [DOI] [PubMed]
23. Huang J, Bu L, Xie J, Chen K, Cheng Z, Li X et al. Effects of nanoparticle size on cellular uptake and liver MRI with PVP-coated iron oxide nanoparticles. ACS Nano. 2010. [DOI]
24. Ge Y, Zhang Y, Xia J, Ma M, He S, Nie F et al. Effect of sur-face charge and agglomerate degree of magnetic iron oxide nanoparticles on KB cellular uptake in vitro. Colloids Surf B Biointerfaces. 2009 Oct;73(2):294–301. [DOI] [PubMed]
25. Naqvi S, Samim M, Abdin M, Ahmed FJ, Maitra A, Prashant C et al. Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress. Int J Nanomedicine. 2010 Nov;5:983–9. [DOI] [PubMed]
26. Soenen SJ, Nuytten N, De Meyer SF, De Smedt SC, De Cuyper M. High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling. Small. 2010 Apr;6(7): 832– 42. [DOI] [PubMed]
27. Haghighat N, Abdolmaleki P, Behmanesh M, Satari M. Stable morphological-physiological and neural protein expression changes in rat bone marrow mesenchymal stem cells treated with electromagnetic field and nitric oxide. Bioelectromagnetics. 2017;38(8):592-601. [DOI]
28. Blank M, Goodman R. DNA is a fractal antenna in elec-tromagnetic fields. Int J Radiat Biol. 2011;87(4):409–15. [DOI] [PubMed]
29. Phillips JL, Haggren W, Thomas WJ, Ishida-Jones T, Adey WR. Magnetic field-induced changes in specific gene transcription. Biochim Biophys Acta. 1992;1132(2):140-4. [DOI]
30. Koziorowska A, SOŁEK P, MAJCHROWICZ L, ROMEROWICZ-MISIELAK M. The impact of electromagnetic fields with frequency of 50 Hz on metabolic activity of cells in vitro. Przegląd Elektrotechniczny. 2017; 5;93(1):161-4.
31. Falone S, Mirabilio A, Carbone MC, Zimmitti V, Di Loreto S, Mariggiò MA et al. Chronic exposure to 50Hz magnetic fields causes a significant weakening of antioxidant defence systems in aged rat brain. Int J Biochem Cell Biol. 2008;40 (12):2762–70. [DOI] [PubMed]
32. Kaiser DF. Theoretical physics and biology: non-linear dynamics and signal amplification—relevant for EMF interaction with biological systems?. InProceedings of the Workshop on Proposed Mechanisms for the Interaction of RF-Signals with Living Matter: Demodulation in Biologi-cal Systems 2006 Sep.
33. Hardell L, Sage C. Biological effects from electromagnetic field exposure and public exposure standards. Biomed Pharmacother. 2008;62(2):104–9. [DOI] [PubMed]
34. Repacholi MH, Greenebaum B. Interaction of static and extremely low frequency electric and magnetic fields with living systems: health effects and research needs. Bioelectromagnetics. 1999; 20 (3): 133–60. [DOI] [PubMed]
35. Pall ML. Electromagnetic fields act via activation of voltage-gated calcium channels to produce beneficial or adverse effects. J Cell Mol Med. 2013;17(8):958–65. [DOI] [PubMed]
36. Jordan A, Wust P, Fahling H, John W, Hinz A, Felix R. Inductive heating of ferrimagnetic particles and magnetic fluids: physical evaluation of their potential for hyperthermia. Int J Hyperthermia. 1993;9(1):51-68. [PubMed]
37. Li W, Liu Y, Qian Z, Yang Y. Evaluation of Tumor Treatment of Magnetic Nanoparticles Driven by Extremely Low Frequency Magnetic Field. Sci Rep. 2017;7:46287. [DOI] [PubMed]

Add your comments about this article : Your username or Email:
Write the security code in the box

Send email to the article author

© 2015 All Rights Reserved | Multidisciplinary Cancer Investigation

Designed & Developed by : Yektaweb